Journal: BMC Cancer

Article Title: Comparison of detection methods and follow-up study on the tyrosine kinase inhibitors therapy in non-small cell lung cancer patients with ROS1 fusion rearrangement

doi: 10.1186/s12885-016-2582-9

Figure Lengend Snippet: The characteristics of patients with ROS1 rearrangement

Article Snippet: The cDNA was used for multiple RT-PCRs that were carried out in an Mx3000p real-time PCR system (Agilent Technologies, California, US) using the ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China).

Techniques: Sequencing, Histopathology, Staining

Journal: BMC Cancer

Article Title: Comparison of detection methods and follow-up study on the tyrosine kinase inhibitors therapy in non-small cell lung cancer patients with ROS1 fusion rearrangement

doi: 10.1186/s12885-016-2582-9

Figure Lengend Snippet: Comparison of IHC, FISH and qRT-PCR in ROS1 rearrangement and non-rearrangement cases ( a – d ). The H&E staining, IHC, FISH and qRT-PCR results of a non-rearrangement case. a The case presented acinar and papillary patterns in H&E staining (200×); b IHC showed no staining in tumor cells (200×); c , d it was confirmed by FISH and qRT-PCR as non-rearrangement, respectively; e – h A case with weak and focal ROS1 IHC staining. e The case presented acinar pattern in H&E staining (200×); f IHC showed weak to moderate focally staining in about 40 % tumor cells (200×); g , h it was also confirmed as non-rearrangement by FISH and qRT-PCR, respectively; i – l A case with diffusely moderate IHC staining. i The case presented papillary and micropapillary patterns in H&E staining (200×); j IHC showed moderate staining with cytoplasmic and focal granular patterns in about 80 % tumor cells (200×); k and l it was proved as ROS1-rearrangement by FISH and qRT-PCR, respectively; m-p A case with diffusely strong IHC staining. m The case presented papillary and micropapillary pattern in H&E staining (200×); n IHC showed diffusely strong staining with cytoplasmic, membrane and granular patterns in about 90 % tumor cells (200×); o , p it was also proved as ROS1 rearrangement by FISH and qRT-PCR

Article Snippet: The cDNA was used for multiple RT-PCRs that were carried out in an Mx3000p real-time PCR system (Agilent Technologies, California, US) using the ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China).

Techniques: Quantitative RT-PCR, Staining, Immunohistochemistry

Journal: BMC Cancer

Article Title: Comparison of detection methods and follow-up study on the tyrosine kinase inhibitors therapy in non-small cell lung cancer patients with ROS1 fusion rearrangement

doi: 10.1186/s12885-016-2582-9

Figure Lengend Snippet: Comparison of FISH and IHC in ROS1 rearrangement detection

Article Snippet: The cDNA was used for multiple RT-PCRs that were carried out in an Mx3000p real-time PCR system (Agilent Technologies, California, US) using the ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China).

Techniques:

Journal: BMC Cancer

Article Title: Comparison of detection methods and follow-up study on the tyrosine kinase inhibitors therapy in non-small cell lung cancer patients with ROS1 fusion rearrangement

doi: 10.1186/s12885-016-2582-9

Figure Lengend Snippet: Characteristic of included cases

Article Snippet: The cDNA was used for multiple RT-PCRs that were carried out in an Mx3000p real-time PCR system (Agilent Technologies, California, US) using the ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China).

Techniques: Histopathology

Journal: BMC Cancer

Article Title: Comparison of detection methods and follow-up study on the tyrosine kinase inhibitors therapy in non-small cell lung cancer patients with ROS1 fusion rearrangement

doi: 10.1186/s12885-016-2582-9

Figure Lengend Snippet: The details of follow-up studies

Article Snippet: The cDNA was used for multiple RT-PCRs that were carried out in an Mx3000p real-time PCR system (Agilent Technologies, California, US) using the ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China).

Techniques: Histopathology

Journal: BMC Cancer

Article Title: Comparison of detection methods and follow-up study on the tyrosine kinase inhibitors therapy in non-small cell lung cancer patients with ROS1 fusion rearrangement

doi: 10.1186/s12885-016-2582-9

Figure Lengend Snippet: The routine protocol for ROS1 arrangements detection in NSCLC. IHC with D4D6 mAb can be preliminary screening tool for ROS1 rearrangement detection in NSCLC, and the IHC cutoff value should be set at 2–3+ cytoplasmic reactivity with diffuse (≥60 % of the tumor cells) distribution or an H-score ≥150; FISH and qRT-PCR should be used as the secondary confirmation in the cases with weak of focal IHC staining

Article Snippet: The cDNA was used for multiple RT-PCRs that were carried out in an Mx3000p real-time PCR system (Agilent Technologies, California, US) using the ROS1 Gene Fusion Detection Kit (Amoy Diagnostics Co., Ltd, Xiamen, China).

Techniques: Quantitative RT-PCR, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Association between epidermal growth factor receptor mutations and the expression of excision repair cross-complementing protein 1 and ribonucleotide reductase subunit M1 mRNA in patients with non-small cell lung cancer

doi: 10.3892/etm.2015.2196

Figure Lengend Snippet: Mutations of the EGFR gene and the expression of ERCC1 and RRM1 mRNA. (A) Positive control, positive sample and negative control of the EGFR gene. (B) Positive control, negative sample and negative control of the EGFR gene. (C) Expression of ERCC1 and RRM1 mRNA. EGFR , epidermal growth factor receptor; ERCC1 , excision repair cross-complementing protein 1; RRM1 , ribonucleotide reductase subunit M1.

Article Snippet: The tumor-associated gene expression detection kit for ERCC1 (ADx-ER01) and RRM1 (ADx-RR01) (Amoy Diagnostics Co., Ltd.) was used to determine the mRNA expression level of these genes using the absolute quantitative method; β-actin was used as the reference gene.

Techniques: Expressing, Positive Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Association between epidermal growth factor receptor mutations and the expression of excision repair cross-complementing protein 1 and ribonucleotide reductase subunit M1 mRNA in patients with non-small cell lung cancer

doi: 10.3892/etm.2015.2196

Figure Lengend Snippet: Association between EGFR mutations, ERCC1 and RRM1 mRNA expression, and patient clinical characteristics.

Article Snippet: The tumor-associated gene expression detection kit for ERCC1 (ADx-ER01) and RRM1 (ADx-RR01) (Amoy Diagnostics Co., Ltd.) was used to determine the mRNA expression level of these genes using the absolute quantitative method; β-actin was used as the reference gene.

Techniques: Expressing, Mutagenesis

Journal: Experimental and Therapeutic Medicine

Article Title: Association between epidermal growth factor receptor mutations and the expression of excision repair cross-complementing protein 1 and ribonucleotide reductase subunit M1 mRNA in patients with non-small cell lung cancer

doi: 10.3892/etm.2015.2196

Figure Lengend Snippet: Association between EGFR mutations and the expression level of ERCC1 mRNA.

Article Snippet: The tumor-associated gene expression detection kit for ERCC1 (ADx-ER01) and RRM1 (ADx-RR01) (Amoy Diagnostics Co., Ltd.) was used to determine the mRNA expression level of these genes using the absolute quantitative method; β-actin was used as the reference gene.

Techniques: Expressing, Mutagenesis

Journal: Experimental and Therapeutic Medicine

Article Title: Association between epidermal growth factor receptor mutations and the expression of excision repair cross-complementing protein 1 and ribonucleotide reductase subunit M1 mRNA in patients with non-small cell lung cancer

doi: 10.3892/etm.2015.2196

Figure Lengend Snippet: Association between the expression levels of ERCC1 and RRM1 mRNA.

Article Snippet: The tumor-associated gene expression detection kit for ERCC1 (ADx-ER01) and RRM1 (ADx-RR01) (Amoy Diagnostics Co., Ltd.) was used to determine the mRNA expression level of these genes using the absolute quantitative method; β-actin was used as the reference gene.

Techniques: Expressing

Journal: The American journal of surgical pathology

Article Title: Nodular Lymphocyte-Predominant Hodgkin Lymphoma With Atypical T Cells: A Morphologic Variant Mimicking Peripheral T-Cell Lymphoma

doi: 10.1097/PAS.0b013e31822832de

Figure Lengend Snippet: Molecular Genetic Features of NLPHL With Atypical T Cells

Article Snippet: T-cell clonality assays were performed using polymerase chain reaction (PCR) according to the manufacturer’s instructions with reagents purchased from InVivoScribe Technologies LLC, San Diego, CA ( TCRγ Gene Clonality Assay for ABI Fluorescence Detection).

Techniques: Polymerase Chain Reaction, Amplification, Southern Blot